产品详情

Mouse monoclonal antibody to PTPRF (APC ). PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function . Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
Host：Mouse
Reacts With：Human,Rat
Immunogen：Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR (accession # P10586). 97% identical in both rat and mouse. > 80% identity with PTPRD and PTPRS. > 50% identity with PTPRM and PTPRK.
Tested Applications：ICC,IF,WB
Application Dilutions：WB: 1:1000
Application Notes：Detects a 85 kDa protein (full length protein is 210 kDa-smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.Experiment Notes: 1 μg/mL of PTPRF was sufficient for detection of LAR/PTPRF in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Conjugation：APC
Clonality：Monoclonal